Development
Cell Line Development

Zencore CHO cell line development platform can rapidly generate high yield, stable cell lines to meet your pressing timeline and ensure an efficient cell culture process development process.

Platform features
  • Rapid stable cell line generation
    • Zencore's proprietary CLD platform generates stable high yield single clones in 3 months, while traditional CLD process takes 5~6 months

  • High throughput screening platform in conjunction with a tailored approach for BsAbs CLD
    • Replacing traditional ELISA with ForteBio Octet has greatly improved the efficiency and accuracy for cell line screening.

    • A high throughput analytical method is implemented to identify cell lines with the high titer and good quality for BsAbs CLD.

  • Compliance documents for BLA
    • Host cell licensure for global R&D and commercialization 

    • Cell line construction process documented compliant with global regulations

    • Supporting global regulatory submission

    • Complete host cell traceability

    • Industrial standard monoclonality guaranteed

    截图-20250114132539

    Single cell printer is used for efficient single cell screening, the monoclonal selection rate is up to 90%, a 5 fold improvement over the traditional limited dilution method.

    Combined with continuous imaging technology of cell culture 96-well plate, we can monitor the growth and division process of single gram cells and provide complete monoclonal traceability. 

  • Stable high producer cell lines for antibodies and recombinant proteins
    • The median titer of mAb was above 5.0 g/L, and the highest titer achieved at 15.0 g/L.

    • The median titer of bispecific antibody was above 5.0 g/L, and the highest titer achieved at 9.0 g/L.

    • The average titer of recombinant protein was above 5.0g/L, and the highest titer achieved at 7.0 g/L.

  • Flexible host cell choice for cell line development
    • Fully adapted ATCC CHO-K1 cells with global licensure for  R&D and commercial production

    • Introducing ECACC CHO-K1 cells

    • Accepting customer-specified host cell platforms development

Major Equipment
Single cell printer
Single cell printer
Cell metric CLD
Cell metric CLD
ForteBio Octet
ForteBio Octet
01/03
×
Contact
arrow_left sharing button
arrow_right sharing button