TRANSIENT TRANSFECTION SERVICE

Transient Expression ServicesTransient Transfection Service

Delivering protein samples close to marketed-product quality quickly for early drug research. The CHO transient expression platform uses the same protein expression system as the CMC development stage, so clients can obtain protein samples approaching marketed drug quality early in R&D.

CHO expression systemmAbs / bispecifics / fusion proteinsPurity >98.0%Endotoxin <0.5 EU/mL
Back to Biologics CDMO Services
200+
Project experience in transient expression and purification
3-4 weeks
From theoretical sequence to protein delivery
>98.0%
Protein purity after 2-3 chromatography purification steps
<0.5EU/ml
Endotoxin level
Platform Advantages

Platform advantagesWhy Choose Zencore

An expression system consistent with CMC, with both fast delivery and strong yields, providing high-quality protein samples for early R&D.

consistent with CMC

An expression system consistent with CMC developmentCMC-aligned Expression System

  • using CHO cellsexpressing the protein so that protein samples used during R&D more closely resemblemarketed drug quality
  • usingPlasmid construction for stable cell linesto express it and assess the target protein's CMC performance earlier
2-8 weeks

Rapid delivery of sufficient proteinRapid & Scalable Delivery

  • Tiered delivery based on project needs, achieving both speed and yield
2 weeks · milligram scale4 weeks · gram scale8 weeks · ten-gram scale
High titer

High expression across multiple molecule typesHigh Titers Across Formats

  • SuitablemAbs, bispecific antibodies and fusion proteins
  • After 2 to 3 chromatography purification steps, protein purity >98.0%, endotoxin <0.5EU/ml
Track Record Data

Historical titer data for different protein typesHistorical Expression Data

Platform historical project titer data (mg/L), with maximum and average titers at a glance.

Platform maximum titer display
(Note: the dashed line marks the average titer)
10007505002500
mAbsSymmetric bispecific antibodiesAsymmetric bispecific antibodiesFusion proteins*
Maximum titer (mg/L)Average titer (dashed line)
*including glycoproteins for vaccine development · data from historical Zencore platform projects
Workflow

Transient expression workflowTransient Transfection Workflow

From theoretical amino acid sequence to GMP-quality protein samples, a standardized workflow ensuring fast delivery.

1

Theoretical amino acid sequence

The client provides the target molecule sequence requirement

2

Gene design

Codon optimization and gene synthesis

3

Expression vector construction

Plasmid construction and large-scale preparation

4

Transient expression

Transfection of CHO cells with DNA-PEI complexes

5

Purification and analysis

2-3 chromatography purification steps, quality control testing

3WEEKS

delivered within three weeks 500mg purity above 98.0% samples

4WEEKS

delivered within four weeks 1g purity above 98.0% samples

Milestones

AchievementsAchievements

200+

Zencore Biologics has completed over 200 transient expression and purification projects, consistently delivering on time.

3-4 weeks

The transient expression service platform provides fast and efficient protein expression. From the client's provision of a theoretical sequence to Zencore's delivery of protein takes only 3-4 weeks.

Rapidly obtaining protein samples close to marketed-product quality

Monoclonal antibody, bispecific or fusion protein? Hand the sequence to Zencore and receive high-purity protein within weeks.

Inquire about transient expression services