CELL LINE DEVELOPMENT

Cell Line DevelopmentCell Line Development

A rapid, high-expression stable cell line development platform that supports fast, platform-based cell culture process development. Zencore can complete construction from protein sequence to monoclonal cell line in just 3 months.

3-month construction cycleMonoclonality rate 90%mAb up to 15.0 g/LGlobal regulatory compliance
Back to Biologics CDMO Services
3months
Sequence - monoclonal cell line (conventionally 5-6 months)
90%
Single Cell Printer effective monoclonality rate
5x+
Monoclonal screening throughput improved versus limiting dilution
15.0g/L
Maximum mAb titer
Platform Features

Platform featuresWhy Our Cell Lines

A triple advantage of timeline, throughput and compliance, creating a fast track from sequence to high-yield stable cell line.

3 months

Short development cyclesShorter Timeline

  • Conventional cell line construction methods require 5-6 monthsCompleting monoclonal cell line development for production; Zencore requires only 3 monthsto complete construction from protein sequence to monoclonal cell line
Conventional methods5-6 months
Zencore platform3 months
High throughput · intelligent

High-throughput screening & bispecific screening enablementHTS & Bispecific Screening

  • use ForteBio Octet Replacing conventional ELISA platforms, greatly improving the throughput and accuracy of cell line screening
  • In bispecific cell line development, specialized analytical methodshighest secreted protein qualitycells are selected
Bispecific clone dual-target titer screening: ranking by dual-target titer to lock in the TOP1 clone
Bispecific clone dual-target titer screening · locking in the TOP1 clone
Traceable

Monoclonal screening with imaging monitoringClonality & Imaging

  • using Single Cell Printer for efficient single-cell screening with an effective monoclonality rate of up to 90%, with throughput improved over limiting dilution by more than 5-fold
  • Binding Cell Metric CLD Continuous imaging of cell culture plates monitors the entire growth and division process of monoclonal cells, providing complete, traceable proof of monoclonality
Monoclonal cell sorting monitoring with Day 0-Day 2 continuous imaging of cell division
Monoclonal sorting with continuous Day 0-2 division imaging monitoring
Workflow

Cell line development workflowDevelopment Workflow

Monoclonal screening begins as early as the Isolate stage, progressively locking in TOP clones, with monoclonal RCB delivered in 3 months.

Cell line development workflow: In silico analysis - Vector construction - Isolate screening - Monoclonal screening of TOP20 RCB - TOP8 Fed-batch - TOP3 monoclonals - PCB banking and stability studies - 2 L BR fed-batch
3 months, from target sequence to monoclonal RCB - Zencore begins monoclonal screening as early as the Isolate stage, progressively narrowing down to TOP3 clones and completing PCB banking and stability studies
High Titer

Stable, high-level expression of various target proteinsStable High Expression

Historical platform data for mAbs, bispecifics and recombinant proteins, with stable titers and batch-to-batch consistency.

mAbsMonoclonal Antibody

15.0g/L · maximum titer
Median ≥5.0 g/LMaximum titer 15.0 g/L

bispecificsBispecific Antibody

9.0g/L · maximum titer
Median ≥5.0 g/LMaximum titer 9.0 g/L

recombinant proteinRecombinant Protein

7.0g/L · maximum titer
Average titer ≥5.0 g/LMaximum titer 7.0 g/L

Horizontal bars drawn to a uniform scale (full bar = 15.0 g/L) - data from historical Zencore platform projects

Hosts & Compliance

Host cell selection and compliance documentationHost Cells & Regulatory Documentation

Multiple host platforms available with complete compliance documentation, supporting global filing and launch.

Multiple options available

Diverse host cell optionsHost Cell Options

  • fully in-house adapted ATCC CHO-K1 cells with global R&D and commercialization rights
  • Introduce ECACC CHO-K1 cells
  • accepting clientother designated host cellsPlatform-based development
Global filings

Compliance documentation supporting project launchRegulatory-ready Documentation

  • holding host cellGlobal R&D and commercializationrights
  • Cell line construction process recordsComplies with global regulations
  • supporting global project filingsCompliance services
  • Comprehensive host cellTraceability documentation
  • industry-standardProof of monoclonalityMaterials
Key Instruments

Main equipmentKey Equipment

the hardware cornerstone for high-throughput monoclonal screening and imaging traceability.

Single Cell Printer single-cell dispensing and sorting instrument

Single Cell Printer

Efficient single-cell printing and sorting with an effective monoclonality rate of up to 90%

Cell Metric CLD clone imaging and tracking system

Cell Metric CLD

Continuous imaging of culture plates to monitor monoclonal growth and division throughout

ForteBio Octet biomolecular affinity analyzer

ForteBio Octet

BLI assays replace conventional ELISA, greatly improving screening throughput and accuracy

3 months, from sequence to high-yield cell line

Monoclonal antibody, bispecific or recombinant protein? Zencore's cell line development platform moves your project rapidly into process and manufacturing stages.

Inquire about cell line development